Journal: Journal of Family & Reproductive Health

Article Title: Toxic Effects of Chronic Exposure to High-Fat Diet and Arsenic on the Reproductive System of the Male Mouse

doi:

Figure Lengend Snippet: Effect of high fat diet and arsenic exposure on plasma LH, FSH and Testosterone levels. Data are presented as Mean ± SEM; n = 12. # P<0.05, ## P<0.01, and ### P<0.001 compared to the LF0. * P<0.05, ** P<0.01, and *** P<0.001 compared to the HF50. LF: Low fat diet; HF; High fat diet.

Article Snippet: Hormonal assessment: Plasma luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone levels have been measured by enzyme-linked immunosorbent assay (ELISA) method with commercial assay kits (DRG Instruments GmbH, Germany) and, hormone detection sensitivity per assay tube of each kit was 0.856 mIU/mL for LH, 1.27 mIU/mL for FSH, and 0.287 nmol/L for testosterone.

Techniques: Clinical Proteomics

Journal: PLoS ONE

Article Title: Effects of castration on atherosclerosis in Yucatan minipigs with genetic hypercholesterolemia

doi: 10.1371/journal.pone.0234131

Figure Lengend Snippet: A . Plasma testosterone levels in orchiectomized (Orx) and control PCSK9 D374Y Yucatan minipigs. *P<0.0001 (t test on AUC). B . Plasma lipid levels on low-fat diet (3 months of age) and on HFHC diet (6, 9, and 12 months of age) was altered in castrated pigs with modestly increased LDL cholesterol (*P = 0.045, t test with Welch’s correction on AUC) and reduced HDL cholesterol (*P = 0.002, t test on AUC). The difference in plasma triglyceride burden over the course of study was not significant (P = 0.06, t test on AUC). C . Representative examples of Sudan red stained thoracic aorta (left), abdominal aorta (middle) and right iliac (right). D-F . Quantification of lesions as fraction of total vessel surface in thoracic aortas (D), abdominal aortas (E), and right iliac artery (F) showing no significant differences between groups. Data from the examples in (C) are marked in red. G . Representative examples of histology of iliac lesions stained with hematoxylin-eosin. Scale bars = 500 μm. H-I . Intimal/lesion areas measured on hematoxylin-eosin stained sections in right iliacs and LADs show no differences between groups. Data from the examples in (G) are marked in red. J . Scoring of aggressive behavior showed significant reduction in orchiectomized pigs. Each animal was observed one to four times. *P = 0.009 (t test with Welch’s correction). Number of data points/animals in each panel are 6 and 8 for the control and orchiectomized groups, respectively. Error bars indicate mean and SEM.

Article Snippet: Plasma testosterone was analyzed in the blood samples collected at 3, 6, 9, and 12 months of age by immuno-chemical methods (Arbor Assays, K032-H1, Michigan 48108 USA).

Techniques: Clinical Proteomics, Control, Staining

Journal: Psychopharmacology

Article Title: Quantification of Ten Neuroactive Steroids in Plasma in Withdrawal Seizure–Prone and –Resistant Mice During Chronic Ethanol Withdrawal

doi: 10.1007/s00213-014-3618-y

Figure Lengend Snippet: Steroids that were simultaneously quantified in the GC-MS assay are indicated with a BOLD asterisk. Note that the potent positive modulators of GABAA receptors (THP, THDOC, androstanediol) are formed by the 2-step reduction of the parent steroids, progesterone, deoxycorticosterone, and testosterone, respectively. Also depicted are the sulfated derivatives of pregnenolone and DHEA (dehydroepiandrosterone), which are negative modulators of GABAA receptors. [DHDOC = dihydrodeoxycorticosterone; THDOC = tetrahydrodeoxycorticosterone; DHP = dihydroprogesterone; THP = tetrahydroprogesterone; DHT = dihydrotestosterone; HSD = hydroxysteroid dehydrogenase]

Article Snippet: This finding is consistent with the reported decrease in plasma testosterone levels in male alcoholics (e.g., Castilla-Garcia et al., 1987; Iturriaga et al., 1999 ; Krüger et al, 2006 ; Maneesh et al., 2006 ; Martinez-Riera et al., 1995 ; Ruusa et al., 1997 ; Sierksma et al., 2004 ; Ylikahri et al., 1980 ).

Techniques: Gas Chromatography-Mass Spectrometry

Journal: Psychopharmacology

Article Title: Quantification of Ten Neuroactive Steroids in Plasma in Withdrawal Seizure–Prone and –Resistant Mice During Chronic Ethanol Withdrawal

doi: 10.1007/s00213-014-3618-y

Figure Lengend Snippet: TIC was obtained for a reference standard mixture (1000 pg of each steroid) using selected ion monitoring (SIM) in negative chemical ionization mode. There was high response and good resolution for all analytes of interest; x-axis depicts range of retention times (min) for elution of the neurosteroid groups (also see Table 1). [Testosterone derivatives = 3α,5α-A-Diol & 3α,5β-A-Diol (androstanediols); Androstenedione derivatives = 3α,5α-A & 3α,5β-A (hydroxyandrostan-17-ones); Progesterone derivatives = 3α,5α-THP (allopregnanolone) & 3α,5β-THP (pregnanolone); Deoxycorticosterone derivatives = 3α,5α-THDOC & 3α,5β-THDOC (tetrahydrodexoycorticosterones)] See Figure 1 for parent steroids.

Article Snippet: This finding is consistent with the reported decrease in plasma testosterone levels in male alcoholics (e.g., Castilla-Garcia et al., 1987; Iturriaga et al., 1999 ; Krüger et al, 2006 ; Maneesh et al., 2006 ; Martinez-Riera et al., 1995 ; Ruusa et al., 1997 ; Sierksma et al., 2004 ; Ylikahri et al., 1980 ).

Techniques:

Journal: Cardiovascular Research

Article Title: Modulation of neointimal lesion formation by endogenous androgens is independent of vascular androgen receptor

doi: 10.1093/cvr/cvu142

Figure Lengend Snippet: Identification of AR in murine vascular cells. AR is expressed in EC (red arrows) and SMC (green arrows) in healthy mouse aorta ( A ). The dashed yellow line indicates the external elastic lamina. AR expression was up-regulated by testosterone (1 × 10 −7 M) in cultured mouse aortic EC ( B ) and SMC ( C ). EC were identified using antibodies against vWF and CD31; SMCs were identified using an antibody against SMA. Nuclei were counter-stained with DAPI.

Article Snippet: Plasma testosterone (DEMEDITEC Diagnostics GmbH, Kiel-Wellsee, Germany), total plasma cholesterol (Olympus Diagnostics Ltd, Watford, UK), and plasma triglyceride (Alpha Laboratories Ltd., Eastleigh, UK) were measured using commercially available kits, in accordance with manufacturer's instructions.

Techniques: Expressing, Cell Culture, Staining

Journal: Cardiovascular Research

Article Title: Modulation of neointimal lesion formation by endogenous androgens is independent of vascular androgen receptor

doi: 10.1093/cvr/cvu142

Figure Lengend Snippet: Characterization of mice with vascular cell-specific AR deletion. Cell-specific AR deletion was confirmed ( A ; n = 4 for each genotype) using PCR on genomic DNA from freshly isolated aortic EC and SMC. Mouse genotypes were confirmed ( B ) with PCR using genomic DNA from ear clip samples. Deletion of vascular AR did not alter circulating testosterone levels ( C ; n = 14–23) or seminal vesicle weight ( D ; n = 8–15) but deletion of AR from EC (VE-ARKO) produced a small increase in systolic blood pressure ( E ). * P < 0.05 ( n = 7–12) by one-way ANOVA plus Bonferroni post-hoc test. (WT = wild-type litter mates carrying floxed-AR; SM-ARKO = AR ablated in SMC, VE-ARKO = AR ablated in EC, SM/VE-ARKO = AR ablated in both EC and SMC.)

Article Snippet: Plasma testosterone (DEMEDITEC Diagnostics GmbH, Kiel-Wellsee, Germany), total plasma cholesterol (Olympus Diagnostics Ltd, Watford, UK), and plasma triglyceride (Alpha Laboratories Ltd., Eastleigh, UK) were measured using commercially available kits, in accordance with manufacturer's instructions.

Techniques: Isolation, Produced

Journal: Cardiovascular Research

Article Title: Modulation of neointimal lesion formation by endogenous androgens is independent of vascular androgen receptor

doi: 10.1093/cvr/cvu142

Figure Lengend Snippet: Testosterone induces relaxation in vascular ARKO arteries. Supra-physiological concentration of testosterone induced vascular relaxation both in femoral ( A ) and mesenteric ( B ) arteries from all genotypes, which was independent of the type of pre-constriction. Vascular ARKO produced no dramatic changes in testosterone-mediated relaxation despite some small differences in relaxation at specific concentrations. * P < 0.05, ** P < 0.01 vs. corresponding WT concentration; # P < 0.05 vs. corresponding VE-ARKO concentration; two-way ANOVA plus Bonferroni post-hoc test. (WT = wild-type litter mates carrying floxed-AR; SM-ARKO = AR ablated in SMC, VE-ARKO = AR ablated in EC, SM/VE-ARKO = AR ablated in both EC and SMC. n = 7–9.)

Article Snippet: Plasma testosterone (DEMEDITEC Diagnostics GmbH, Kiel-Wellsee, Germany), total plasma cholesterol (Olympus Diagnostics Ltd, Watford, UK), and plasma triglyceride (Alpha Laboratories Ltd., Eastleigh, UK) were measured using commercially available kits, in accordance with manufacturer's instructions.

Techniques: Concentration Assay, Produced

Journal: Cardiovascular Research

Article Title: Modulation of neointimal lesion formation by endogenous androgens is independent of vascular androgen receptor

doi: 10.1093/cvr/cvu142

Figure Lengend Snippet: Castration reduces vascular AR expression. Plasma testosterone concentrations ( A ), seminal vesicle weight ( B ), and AR expression in lesion-bearing femoral arteries ( C ) were reduced following castration. ** P < 0.01. Data were analysed by Student's t -test. ( A : n = 9–11; B : n = 8–15; C : n = 9).

Article Snippet: Plasma testosterone (DEMEDITEC Diagnostics GmbH, Kiel-Wellsee, Germany), total plasma cholesterol (Olympus Diagnostics Ltd, Watford, UK), and plasma triglyceride (Alpha Laboratories Ltd., Eastleigh, UK) were measured using commercially available kits, in accordance with manufacturer's instructions.

Techniques: Expressing, Clinical Proteomics